![]() ![]() Lysis of the bacteriophage is indicated by the formation of a zone of clearing or plaque within the lawn of bacteria. ![]() Escherichia coli), the phage attaches the bacterial cell, replicate inside it, and kills it during its lytic release. T4 phage) is spread over the lawn of susceptible bacterial cells (e.g. ![]() When a suspension of an infective phage (e.g. Results Principle of Phage Plaque Assay.Overlaying Plate with Phage-Agar Mixture.Preparation of Stock Solution by serial dilution.Procedure for Bacteriophage Plaque Assay.Currently, its modified procedure is being used for the determination of titer of many different animal viruses too. Only viruses that cause visible damage to cells can be assayed in this way. Plaque assay was first developed to calculate the titers of bacteriophage stocks. Plaque assay is one of the widely used approaches for determining the quantity of infectious virus in a sample. Using phages to treat bacterial infections was developedback in the 1920s and 1930s in Eastern Europe and the Soviet Union. Temperate Phage: Temperate phages are able to enter a non-lytic prophage state.Īs the world is struggling with the problems of increase in antimicrobial resistance, various research are underway to evaluate applications of phages to treat bacterial infections (as a replacement of antibiotic therapy).Virulent (lytic phage): Growth of virulent phage in susceptible bacteria destroys the host cells and produces many copies of themselves.Bacteriophage (phage for short) are classified into two major groups on the basis of their mode of propagation: A typical phage contains “head” (containing DNA embedded in a protein envelope), “neck”and a protein “tail” (for adsorption to the receptor present in a bacterial cell surface). Bacteriophage “bacteria eaters” are infectious agents that replicate as obligate intracellular parasites in bacteria but in extracellular environment they are metabolically inert. ![]()
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